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The Xenopus laevis mitochondrial protein mtDBP-C cooperatively folds the DNA in vitro
B Mignotte1, E Delain, D Rickwood
1Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France.
The EMBO Journal
|December 1, 1988
Abstract:
The binding of the Xenopus laevis mitochondrial protein mtDBP-C to DNA was studied by equilibrium density banding, agarose gel electrophoresis and electron microscopy. The results obtained show that the mtDBP-C binds cooperatively to DNA irrespective of whether the DNA is supercoiled, relaxed or linear and it induces the formation of superhelical turns locally leading to the formation of a highly folded structure. It appears that this protein could be involved in the compaction of DNA in the mitochondrial nucleoid.