CRISPR in livestock: From editing to printing

Area of Science:

• Agricultural Science
• Biotechnology
• Animal Science

Background:

• The CRISPR revolution has enabled precise genome editing in large animals for livestock and biomedical applications.
• Applying CRISPR technology to large animals presents unique challenges beyond the editing process itself.

Purpose of the Study:

• To review the latest advances and technical issues in CRISPR genome editing for large animals.
• To discuss CRISPR applications for enhancing livestock production and addressing global food demands.
• To explore the ethical considerations surrounding CRISPR technology in animals.

Main Methods:

• Review of current CRISPR technologies and their application in large animal models.
• Analysis of critical steps including genome editing, embryo production, microinjection/electroporation, cryopreservation, and embryo transfer.
• Discussion of case studies and potential applications in livestock improvement.

Main Results:

• CRISPR technology is advancing rapidly, but successful implementation in large animals requires overcoming significant technical hurdles.
• Optimizing embryo production, microinjection, cryopreservation, and embryo transfer are crucial for efficient genome editing outcomes.
• CRISPR offers potential benefits for livestock efficiency, environmental impact reduction, pest control, animal welfare, and health.

Conclusions:

• While technical challenges persist, CRISPR genome editing holds immense promise for transforming livestock and biomedical fields.
• Addressing ethical, societal, and scientific considerations is essential for the responsible advancement and adoption of CRISPR in large animals.
• The future of CRISPR in large animals necessitates a balanced approach, weighing benefits against risks and fostering public consensus.