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Published on: May 13, 2019
Transcription Inhibition by PNA-Induced R-Loops
Boris P Belotserkovskii1, Sum-Yan Ng1, Philip C Hanawalt2
1Department of Biology, Stanford University, Stanford, CA, USA.
Abstract:
R-loops are structures consisting of an RNA-DNA duplex and an unpaired DNA strand. They can form during transcription upon nascent RNA "threadback" invasion into the DNA duplex to displace the non-template DNA strand. R-loops occur naturally in all kingdoms of life, and they have multiple biological effects. Therefore, it is of interest to study the artificial induction of R-loops and to monitor their effects in model in vitro systems to learn mechanisms. Here we describe transcription blockage in vitro by R-loop formation induced by peptide nucleic acid (PNA) binding to the non-template DNA strand.
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