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In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA)
Takehiko Shiraishi1, Mahdi Ghavami2, Peter E Nielsen2
1Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. shiraishiplant@gmail.com.
Abstract:
Cellular delivery methods are a prerequisite for cellular studies with PNA. This chapter describes PNA cellular delivery using cell-penetrating peptide (CPP)-PNA conjugates and transfection of PNA-ligand conjugates mediated by cationic lipids. Furthermore, two endosomolytic procedures employing chloroquine treatment or photochemical internalization (PCI) for significantly improving PNA delivery efficacy are described.
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