Related Experiment Video

Updated: Dec 27, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

4.3K

Characterization of Plant 3D Chromatin Architecture, In Situ Hi-C Library Preparation, and Data Analysis

Pengfei Dong1, Silin Zhong2

  • 1State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Sha Tin, Hong Kong, China.

Methods in Molecular Biology (Clifton, N.J.)
|February 24, 2020
PubMed

Keywords:
A/B compartmentDomainIn situ Hi-CLocal compartmentLoopPlant chromatin 3D architecture

More Related Videos

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

411.0K
Capturing Chromosome Conformation Across Length Scales10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

4.0K

Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.0K
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
8.7K
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
17.7K