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Meiotic gatekeeper STRA8 regulates cell cycle by interacting with SETD8 during spermatogenesis
Changmin Niu1,2, Jiaqian Guo1,2, Xueyi Shen1,2
1Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou, China.
Journal of Cellular and Molecular Medicine
|February 25, 2020
Summary
Stimulated By Retinoic Acid Gene 8 (STRA8) and SETD8 protein interaction regulates spermatogenesis. These genes show cell cycle-dependent expression and may control germ cell development via ubiquitination pathways.
Area of Science:
- Reproductive Biology
- Molecular Genetics
- Cell Biology
Background:
- Stimulated By Retinoic Acid Gene 8 (STRA8) is a retinoic acid-induced gene crucial for male germ cell development.
- SETD8 is a methyltransferase implicated in various cellular processes.
Purpose of the Study:
- To investigate the interaction between STRA8 and SETD8.
- To elucidate their roles in spermatogenesis and cell cycle regulation.
Main Methods:
- Yeast two-hybrid assay
- Co-immunoprecipitation
- Promoter activity assays
- Analysis of gene expression in mouse testis and cell lines
Main Results:
- STRA8 and SETD8 interact and mutually regulate each other's transcription.
- Both proteins exhibit cell cycle-dependent expression in germline cells.
- SETD8, H4K20me1, and PCNA co-localize with STRA8 in mouse spermatogonia.
- Abnormal expression of cell cycle and ubiquitination factors was observed in STRA8 models.
Conclusions:
- STRA8 and SETD8 interaction is vital for spermatogenesis.
- They may regulate germ cell development through the Cdl4-Clu4A-Ddb1 ubiquitinated degradation axis in a PCNA-dependent manner.
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