Visualizing the macroscale spatial distributions of biofilms in complex flow channels using industrial computed...

Area of Science:

• Biotechnology
• Environmental Science
• Engineering

Background:

• Biofilms in enclosed pipelines cause significant problems.
• Optical imaging struggles to monitor biofilm distribution at the macroscale.

Purpose of the Study:

• To develop a noninvasive method for visualizing and quantifying macroscale biofilm distribution in drip irrigation emitters.
• To investigate the influence of hydrodynamic characteristics on biofilm distribution.
• To identify emitter design defects contributing to biofilm accumulation.

Main Methods:

• Utilized industrial computed tomography (ICT) in conjunction with a contrast agent.
• Applied the technique to three types of drip irrigation emitters.
• Analyzed the macroscale spatial distributions and biomass of biofilms.

Main Results:

• ICT successfully visualized and quantified macroscale biofilm distributions.
• Biofilm distribution was influenced by complex hydrodynamic characteristics within emitter channels.
• Biofilms predominantly adhered to lateral and medial surfaces, decreasing in biomass along flow paths.
• Emitter structural design defects were identified based on biofilm distribution patterns.

Conclusions:

• ICT with a contrast agent offers a visual and effective method for studying biofilm formation mechanisms.
• This approach can guide the optimization of flow channel structures to mitigate biofilm accumulation.
• The findings contribute to improved irrigation system design and maintenance.