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Related Experiment Video

Updated: Dec 27, 2025

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Nanoengineered Osteoinductive Bioink for 3D Bioprinting Bone Tissue.

David Chimene1, Logan Miller1, Lauren M Cross1

  • 1Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, Texas 77843, United States.

ACS Applied Materials & Interfaces
|February 25, 2020
PubMed
Summary

A novel nanoengineered ionic covalent entanglement (NICE) bioink enables 3D bioprinting of bone tissue. This advanced bioink promotes stem cell differentiation and extracellular matrix deposition, paving the way for patient-specific bone regenerative medicine.

Keywords:
bone bioprintinghydrogelsionic-covalent reinforcementnanomaterialsosteoinductive bioinks

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Developing advanced bioinks is crucial for 3D bioprinting patient-specific constructs in regenerative medicine.
  • Existing bioinks face challenges in printability, structural stability, biodegradability, and bioactivity.

Purpose of the Study:

  • To develop and characterize a novel nanoengineered ionic covalent entanglement (NICE) bioink for 3D bone bioprinting.
  • To evaluate the potential of NICE bioink to support cell viability, induce osteogenic differentiation, and facilitate bone tissue formation.

Main Methods:

  • Formulation of NICE bioink with controlled printability and mechanical properties.
  • 3D bioprinting of cell-laden scaffolds using human mesenchymal stem cells (hMSCs).
  • Assessment of cell-induced scaffold remodeling, extracellular matrix deposition, and endochondral differentiation via RNA-sequencing.

Main Results:

  • NICE bioink demonstrated excellent printability and enabled fabrication of mechanically resilient, cellularized 3D structures.
  • Encapsulated hMSCs underwent cell-induced remodeling and deposited extracellular matrix proteins over 60 days.
  • NICE bioink promoted endochondral differentiation of hMSCs without osteoinductive agents, confirmed by transcriptome analysis.

Conclusions:

  • The developed NICE bioink is a promising osteoinductive biomaterial for 3D bioprinting of bone tissue.
  • NICE bioink facilitates the formation of mineralized extracellular matrix in growth factor-free conditions.
  • This technology holds potential for fabricating patient-specific bone scaffolds for clinical applications in craniomaxillofacial defect repair.