Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Improved methods of producing precipitating aspergillus antigens.

R Burrell, C K Thomas

Annals of Allergy

|March 1, 1977

Summary

Researchers developed better methods for producing Aspergillus fumigatus antigens for sensitive and reproducible counterimmunoelectrophoresis (CIEP) tests. Key factors influencing antigen production were identified and optimized for improved diagnostic performance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular typing of enteroviruses: comparing 5'UTR, VP1 and whole genome sequencing methods.

Pathology·2022

Same author

Identification and classification of involuntary leg muscle contractions in electromyographic records from individuals with spinal cord injury.

Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology·2014

Same author

Human spinal cord injury: motor unit properties and behaviour.

Acta physiologica (Oxford, England)·2013

Same author

Studies to further investigate the inhibition of human liver microsomal CYP2C8 by the acyl-β-glucuronide of gemfibrozil.

Drug metabolism and disposition: the biological fate of chemicals·2011

Same author

Use of effluent water in sewage treatment plants.

Sewage works journal·2010

Same author

Fatigue in human thenar muscles paralysed by spinal cord injury.

Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology·2010

Area of Science:

Medical Mycology
Immunodiagnostics
Biotechnology

Background:

Aspergillus fumigatus is an opportunistic fungal pathogen causing significant morbidity and mortality.
Accurate and reproducible diagnostic methods are crucial for timely treatment of invasive aspergillosis.
Counterimmunoelectrophoresis (CIEP) is a valuable technique for detecting fungal antigens but requires optimized antigen preparations.

Purpose of the Study:

To optimize methods for producing Aspergillus fumigatus antigens for enhanced sensitivity and reproducibility in CIEP assays.
To investigate critical factors affecting antigen yield and quality for improved diagnostic utility.

Main Methods:

Systematic investigation of various parameters influencing antigen production.
Evaluation of different casein hydrolysate sources as nitrogen sources.
Optimization of incubation methods, freeze-pressing, lyophilization, culture age, and carbon-to-nitrogen ratios.

Main Results:

Identified specific conditions that significantly improve the sensitivity and reproducibility of Aspergillus fumigatus antigens in CIEP.
Demonstrated the impact of nutrient sources, post-run slide handling, and sample processing on antigen performance.
Established optimal parameters for antigen production, leading to more reliable diagnostic results.

Conclusions:

The study successfully developed improved antigen production methods for Aspergillus fumigatus.
Optimized antigen preparations enhance the diagnostic accuracy and reliability of CIEP for aspergillosis.
These findings contribute to the development of more effective diagnostic tools for fungal infections.

Related Experiment Videos