Magnetic solid phase extraction followed with LC-MS/MS for determination of glimepiride in beagle dog plasma and its...

In this study, the core-shell polydopamine-coated magnetic nanomaterials were synthesized with one-step approach and applied as magnetic solid phase extraction adsorbents combined with LCMS/MS for quantification of the diabetes drug glimepiride in beagle dog plasma. The Fe₃O₄@PDA nanomaterials have strong magnetic response, good dispersibility in aqueous solution, and numerous binding sites for π-π interaction and hydrogen bonding with glimepiride. Based on these merits, glimepiride could be quickly extracted and separated from plasma within 10min. The established method has good linearity (1-2000ng•mL^-1), low quantification limit (1ng•mL^-1), good precision (RSDs≤12 %), and satisfactory extraction recovery (71.20-85.70 %). Moreover, we successfully applied this method to the bioequivalence study of generic and innovator products of glimepiride in beagle dog plasma.