Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Engineering antibody affinity and specificity.

D M Webster1, S Roberts, J C Cheetham

  • 1Laboratory of Molecular Biophysics, Oxford University, UK.

International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The antibody combining site: retrospect and prospect.

Immunology today·2014
Same author

Total parenteral nutrition vs oral diet in autologous hematopoietic cell transplant recipients.

Bone marrow transplantation·2003
Same author

Probabilistic methods in BCI research.

IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society·2003
Same author

The physiology of the disc and its clinical relevance.

The Journal of bone and joint surgery. British volume·2003
Same author

Use of active barriers to reduce eutrophication problems in urban lakes.

Water science and technology : a journal of the International Association on Water Pollution Research·2003
Same author

Time-resolved areal-density measurements with proton spectroscopy in spherical implosions.

Physical review letters·2003
Same journal

Abstracts of the 18th UICC International Cancer Congress. 30 June-5 July 2002. Oslo, Norway.

International journal of cancer. Supplement = Journal international du cancer. Supplement·2002
Same journal

A postscript to the international workshop on assessing health-related quality of life in children with cancer.

International journal of cancer. Supplement = Journal international du cancer. Supplement·2000
Same journal

Obstacles and opportunities for the use of health-related quality-of-life assessment in pediatric cancer clinical trials (discussion).

International journal of cancer. Supplement = Journal international du cancer. Supplement·2000
Same journal

Measuring health-related quality of life in pediatric oncology patients: a brief commentary on the state of the art of measurement and application (discussion).

International journal of cancer. Supplement = Journal international du cancer. Supplement·2000
Same journal

Measuring health-related quality of life in childhood cancer: lessons from the workshop (discussion).

International journal of cancer. Supplement = Journal international du cancer. Supplement·2000
Same journal

Feasibility of implementing health promotion interventions to improve health-related quality of life.

International journal of cancer. Supplement = Journal international du cancer. Supplement·2000
See all related articles

Researchers used computational methods and mutagenesis to understand antibody-antigen binding. This knowledge allows for altering antibody affinity and specificity, aiding in drug design.

Area of Science:

  • Immunology
  • Computational Biology
  • Biochemistry

Background:

  • Antibody-antigen interactions are crucial for immune responses and therapeutic applications.
  • Understanding the molecular basis of antibody binding is key to engineering antibodies with desired properties.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying antibody-antigen recognition and binding.
  • To explore methods for altering antibody affinity and specificity using computational and experimental approaches.

Main Methods:

  • Utilized ab initio calculations, knowledge-based prediction, and molecular graphics.
  • Employed site-directed mutagenesis experiments on an anti-lysozyme antibody (Gloop2).

Main Results:

Related Experiment Videos

  • Identified the roles of electrostatic hydrogen bonding, patch matching, and van der Waals forces in binding.
  • Demonstrated the ability to modify antibody affinity and specificity through targeted mutations.

Conclusions:

  • Molecular details of antibody-antigen recognition can be probed using a combination of computational and experimental techniques.
  • Engineered antibodies, including reconstructed, chimeric, and catalytic antibodies, hold potential for drug design.