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Related Experiment Video

Updated: Dec 27, 2025

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

A CRISPR Test for Rapidly and Sensitively Detecting Circulating EGFR Mutations.

Jen-Hui Tsou¹, Qixin Leng¹, Feng Jiang¹

¹Department of Pathology, University of Maryland School of Medicine, 10 S. Pine St. Baltimore, MD 21201, USA.

Diagnostics (Basel, Switzerland)

|February 26, 2020

Summary

A new CRISPR-Cas12a test rapidly and sensitively detects EGFR mutations in cell-free DNA. This method offers a simpler, faster alternative to current techniques for personalized cancer therapy.

Keywords:

CRISPR EGFR cancer liquid biopsy mutations plasma

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Last Updated: Dec 27, 2025

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

EGFR mutations in cell-free DNA guide personalized cancer therapy.
Current detection methods are costly, slow, and moderately sensitive.
CRISPR technology offers a promising avenue for nucleic acid detection.

Purpose of the Study:

To develop a simple and sensitive CRISPR-Cas12a test for detecting circulating EGFR mutations in plasma.
To compare the performance of CRISPR-Cas12a with droplet digital PCR (ddPCR).

Main Methods:

CRISPR-Cas12a system and ddPCR were used to test serially diluted DNA samples with EGFR mutations (L858R, T790M).
Both methods were applied to plasma samples from lung cancer patients and cancer-free individuals.

Main Results:

CRISPR-Cas12a detected L858R and T790M mutations with a 0.005% limit of detection in under three hours.
ddPCR had a 0.05% limit of detection and took over five hours.
CRISPR-Cas12a identified EGFR mutations in patient plasma, correlating with tissue biopsy results, while ddPCR showed limitations.

Conclusions:

The CRISPR-Cas12a system demonstrates rapid and sensitive detection of circulating EGFR mutations.
This technology holds potential for prognostic and therapeutic implications in cancer care.