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Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola

Jia Zhou1, Yuhua Wang1, Xiaofei Liang2

  • 1Key Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, China.

Viruses
|February 26, 2020
PubMed

Insights

Researchers discovered a new (+) single-stranded RNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), in fungi. This virus, potentially residing in mitochondria, represents a new genus within the Botourmiaviridae family.

Area of Science:

  • Mycology
  • Virology
  • Molecular Biology

Background:

  • Fungal viruses, or mycoviruses, can influence host traits and ecosystem dynamics.
  • The diversity of mycoviruses, particularly (+) ssRNA viruses, is continually expanding.
  • Understanding viral genomes and host interactions is crucial for fungal biology.

Purpose of the Study:

  • To report the discovery and characterization of a novel mycovirus.
  • To determine the genomic features and phylogenetic placement of the new virus.
  • To investigate the subcellular localization of the mycovirus within its host.

Main Methods:

  • Virus isolation and genome sequencing.
  • Bioinformatic analysis of the viral genome and open reading frame (ORF).
  • Cellular fractionation assays to determine RNA localization.
  • Phylogenetic analysis to establish taxonomic relationships.

Main Results:

  • A novel (+) single-stranded RNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), was identified from *Phoma matteucciicola*.
  • The PmOLV1 genome is 2603 nucleotides with a single ORF encoding an RNA-dependent RNA polymerase (RdRp).
  • PmOLV1 RNAs showed enrichment in mitochondria, and phylogenetic analysis placed it in the proposed genus *Penoulivirus* within the *Botourmiaviridae* family.

Conclusions:

  • PmOLV1 is a newly identified mycovirus belonging to the *Botourmiaviridae* family.
  • The virus's potential mitochondrial association warrants further investigation.
  • This discovery expands the known diversity of mycoviruses and their evolutionary relationships.
Keywords:
NarnaviridaePhoma matteucciicolamycovirusourmiavirus

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