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Protein conformational changes induced by guanidine at predenaturational concentrations
1Section for Chemistry and Biochemistry, Faculty of Medicine, University of Naples, Italy.
Abstract:
The nature of the molecular event that apomyoglobin undergoes at predenaturational concentrations of guanidine has been investigated by means of steady-state and multifrequency phase and modulation fluorometry. The results have been compared to those observed for liver alcohol dehydrogenase. From these studies has been hypothesized a different susceptibility of the distinct elements of secondary, super-secondary, and tertiary structure towards the denaturing action of guanidine at predenaturational concentrations.
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