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Long noncoding RNA HOXA-AS2 acts as an oncogene by targeting miR-145-3p in human non-small cell lung cancer

Y-B Shi1, S-L Liu, X-R Mou

  • 1Department of Cardiothoracic Surgery, Yantai Mountain Hospital, Yantai, China. 296233234@qq.com.

European Review for Medical and Pharmacological Sciences
|February 26, 2020
PubMed
Abstract

Insights

Long non-coding RNA HOXA-AS2 (Hoxa cluster antisense RNA 2) promotes non-small cell lung cancer (NSCLC) metastasis by targeting microRNA-145-3p (miR-145-3p), suggesting HOXA-AS2 as a potential therapeutic target for NSCLC.

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in various diseases, including cancers.
  • The specific role of lncRNA HOXA-AS2 in non-small cell lung cancer (NSCLC) development requires further elucidation.

Purpose of the Study:

  • To investigate the role of lncRNA HOXA-AS2 in the development of NSCLC.
  • To explore the underlying molecular mechanism involving microRNA-145-3p (miR-145-3p).

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) for HOXA-AS2 expression analysis.
  • In vitro assays (Wound healing, Transwell) to assess NSCLC cell migration and invasion.
  • Luciferase reporter gene assay to confirm the interaction between HOXA-AS2 and miR-145-3p.

Main Results:

  • HOXA-AS2 expression was significantly upregulated in NSCLC tissues compared to adjacent tissues.
  • High HOXA-AS2 expression correlated with reduced disease-free survival in NSCLC patients.
  • Silencing HOXA-AS2 inhibited NSCLC cell migration and invasion in vitro.
  • HOXA-AS2 directly targets miR-145-3p in NSCLC.

Conclusions:

  • HOXA-AS2 promotes NSCLC cell migration and invasion by targeting miR-145-3p.
  • HOXA-AS2 represents a potential therapeutic target for NSCLC treatment.

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