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Updated: Dec 27, 2025

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Induced Self-aspiration Electrospray Ionization Mass Spectrometry for Flexible Sampling and Analysis
Qian Zhang1,2, Yanping Zhu1, Yuan Tian1
1Division of Advanced Manufacturing, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China.
Abstract:
Electrospray ionization (ESI) operating in pulse mode can enhance the utilization efficiency of the electrospray ions by a mass spectrometer. Herein, a novel ionization technique called induced self-aspiration-electrospray ionization (ISA-ESI) was developed based on self-aspiration sampling and capacitive induction. The sample solution polarized in a strong electric field was pulsed drawn into a capillary that was connected to a subambient chamber. The sample solution with polarized ions forms a charged liquid column, which can initiate an electrospray when reaching the capillary outlet. In addition to the self-aspiration ability, the use of a constant high voltage supply and no electrical contact with the solution can also simplify the sampling and ionization operation, enabling a convenient ESI mass spectrometry analysis. The developed ISA-ESI source has been used for multidimensional monitoring of chemical reactions as well as liquid extraction surface analysis of plant tissues. It was expected that this special ionization method could be extended to automated high-throughput ESI-MS analysis.
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