Related Experiment Video
Updated: Dec 27, 2025

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
Published on: March 4, 2021
Draft Genome Resource for Macrophomina phaseolina Associated With Charcoal Rot in Sorghum
Neeraj Purushotham1, Ashley Jones2, Barsha Poudel1
1The Centre for Crop Health, University of Southern Queensland, Toowoomba, Queensland, Australia.
Abstract:
Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable resource to aid future studies in population genomics and molecular diagnostic marker development for rapid detection of the pathogen.

