Monocular and binocular opto-locomotor reflex biases for random dot motion in mice

We investigated the relationship between eyes receiving visual input of large field translating random dot motion and subsequent reflexive changes in running direction in mice. The animals were head-fixed running on a Styrofoam ball and the opto-locomotor reflex (OLR) was measured in response to 2 s of dots patterns moving horizontally to the left or right. We measured the OLR in conditions with both eyes open (binocular) and one eye closed (monocular). When we covered the right or left eye in the monocular condition, we found reflexive behavior to be delayed for a few hundred milliseconds to leftward or rightward motion, respectively. After this delay, the bias disappeared and reflexive behavior was similar to responses to motion under binocular conditions. These results might be explained by different contributions of subcortical and cortical visual motion processing pathways to the OLR. Furthermore, we found no evidence for nonlinear interactions between the two eyes, because the sum of the OLR of the two monocular conditions was equal in amplitude and temporal characteristics to the OLR under binocular conditions.