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Exogenous hydrogen sulfide inhibits human melanoma cell development via suppression of the PI3K/AKT/ mTOR pathway

Qing Xiao1, Jiayi Ying1, Zhuhui Qiao1

  • 1Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China.

Journal of Dermatological Science
|February 27, 2020
PubMed
Abstract

Insights

Hydrogen sulfide (H2S) inhibits melanoma cell growth, migration, and division. This gasotransmitter may offer a new therapeutic strategy for melanoma by targeting the PI3K/AKT/mTOR pathway.

Area of Science:

  • Oncology
  • Biochemistry
  • Molecular Biology

Background:

  • Melanoma is an aggressive skin cancer with limited therapeutic options.
  • Hydrogen sulfide (H2S), a gasotransmitter, influences cancer progression.
  • The role of H2S in human melanoma is not well understood.

Purpose of the Study:

  • To investigate the effects of exogenous H2S on human melanoma cells.
  • To elucidate the molecular mechanisms underlying H2S action in melanoma.

Main Methods:

  • Human melanoma cell lines (A375, SK-MEL-28) were treated with a hydrogen sulfide donor (NaHS).
  • Cell proliferation, migration, and apoptosis were assessed using CCK-8, scratch assays, and flow cytometry.
  • Western blotting and transmission electron microscopy (TEM) were used to analyze protein expression and cellular changes.

Main Results:

  • NaHS treatment inhibited melanoma cell proliferation, migration, and division.
  • H2S induced apoptosis and autophagy in melanoma cells.
  • NaHS decreased the expression of p-PI3K, p-Akt, and mTOR; IGF-1 reversed these effects.

Conclusions:

  • Exogenous hydrogen sulfide inhibits human melanoma cell development by suppressing the PI3K/AKT/mTOR pathway.
  • Hydrogen sulfide presents a potential therapeutic avenue for melanoma treatment.
Keywords:
Hydrogen sulfideMelanomaPI3K/AKT/ mTOR pathway

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