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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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What is Genetic Engineering?00:49

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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Dec 27, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Genetically Engineering the Nervous System with CRISPR-Cas.

Alfredo Sandoval1, Hajira Elahi1, Jonathan E Ploski2

  • 1School of Behavioral and Brain Sciences and the Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75080.

Eneuro
|February 27, 2020
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas gene editing offers powerful tools for understanding the brain. This technology enables rapid creation of models for neurologic diseases, advancing neuroscience research.

Keywords:
CRISPRCasCpf1brainnervous systemneuron

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Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Mammalian brain complexity poses challenges to understanding neuronal functions.
  • Next-generation sequencing and gene-editing technologies are advancing molecular neuroscience.
  • CRISPR-Cas system is a key genetic tool for genome manipulation.

Purpose of the Study:

  • Review recent developments in CRISPR-mediated genome engineering.
  • Highlight CRISPR applications in the normal and diseased nervous system.
  • Discuss limitations and future directions of CRISPR technology in neuroscience.

Main Methods:

  • Overview of the canonical CRISPR-Cas system function.
  • Functional review of CRISPR adaptations for genetic interrogation.
  • Emphasis on applications in neurologic disease research.

Main Results:

  • CRISPR-Cas enables rapid generation of in vitro and transgenic disease models.
  • Facilitates genetic interrogation of the nervous system.
  • Advances understanding of complex neurologic diseases.

Conclusions:

  • CRISPR-Cas technology is revolutionizing neuroscience research.
  • Future modifications promise deeper insights into brain function and disease.
  • CRISPR is crucial for deciphering complex neurologic functions.