Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative evaluation of EUCAST RAST and QuickMIC for rapid antimicrobial susceptibility testing of carbapenem-resistant organisms directly from positive blood cultures.

Microbiology spectrum·2026
Same author

Droplet microfluidics with image texture quantification for detection of rare antibiotic-resistant subpopulations from bloodstream infections.

NPJ digital medicine·2026
Same author

Rapid AST in practice - a workflow analysis of the QuickMIC<sup>®</sup> rapid AST system at multiple clinical laboratories in Europe.

Frontiers in cellular and infection microbiology·2026
Same author

Correction: Enhancing glioblastoma therapy: unveiling synergistic anticancer effects of Onalespib - radiotherapy combination therapy.

Frontiers in oncology·2026
Same author

Antibiotic use and gut microbiome composition links from individual-level prescription data of 14,979 individuals.

Nature medicine·2026
Same author

CombiCTx: screening diffusion gradients of anti-cancer drug combinations.

Lab on a chip·2026

Related Experiment Video

Updated: Dec 27, 2025

Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.8K

A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing.

Pikkei Wistrand-Yuen1, Christer Malmberg2,1, Nikos Fatsis-Kavalopoulos2

  • 1Gradientech AB, Uppsala, Sweden.

Mbio
|February 27, 2020
PubMed
Summary
This summary is machine-generated.

A new multiplex fluidic chip offers rapid phenotypic antibiotic susceptibility testing (AST) for bacteria. This technology provides results within 2-4 hours, improving patient outcomes and guiding targeted antimicrobial therapy.

Keywords:
antibiotic susceptibility testingclinical isolatesfluidic chipmicrofluidicsmultiplex

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.7K
Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.2K

Related Experiment Videos

Last Updated: Dec 27, 2025

Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.8K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.7K
Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.2K

Area of Science:

  • Microfluidics
  • Bacteriology
  • Clinical Microbiology

Background:

  • Severe infections often receive inappropriate empirical antibiotic treatment due to slow diagnostic methods.
  • Emerging antibiotic resistance necessitates faster methods for effective antimicrobial therapy.
  • Traditional antibiotic susceptibility testing (AST) methods have turnaround times of 2-3 days.

Purpose of the Study:

  • To develop a multiplex fluidic chip for rapid phenotypic antibiotic susceptibility testing (AST) of bacteria.
  • To enable faster detection of bacterial antibiotic susceptibility to improve clinical outcomes.
  • To reduce mortality and the overuse of broad-spectrum antibiotics.

Main Methods:

  • A multiplex fluidic chip was designed for bacterial microcolony growth monitoring in antibiotic gradients.
  • Clinical isolates of *Escherichia coli*, *Klebsiella pneumoniae*, and *Staphylococcus aureus* were tested.
  • Automated image analysis tracked microcolony growth to determine Minimum Inhibitory Concentration (MIC) values.

Main Results:

  • Stable MIC values were obtained within 2 to 4 hours.
  • The chip demonstrated 86% categorical agreement with reference broth microdilution methods.
  • Rapid phenotypic AST results were achieved for common bacterial pathogens.

Conclusions:

  • The developed multiplex fluidic chip enables rapid phenotypic antibiotic susceptibility testing (AST) within hours.
  • This technology can facilitate early initiation of appropriate antibiotic therapy for severe infections.
  • Faster AST results have the potential to improve patient outcomes and combat antibiotic resistance.