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MiR-195-5p Inhibits Malignant Progression of Cervical Cancer by Targeting YAP1

Xiaomin Liu1, Yi Zhou1, Yu-E Ning1

  • 1Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang 110000, People's Republic of China.

Oncotargets and Therapy
|February 27, 2020
PubMed
Abstract

Insights

MicroRNA-195-5p acts as a tumor suppressor in cervical cancer by inhibiting Yes-associated protein 1 (YAP1). This finding supports targeted therapy for cervical cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Cervical cancer exhibits reduced levels of microRNA-195 (miR-195).
  • Previous studies indicate miR-195 upregulation suppresses cervical cancer cell growth and induces cell cycle arrest.

Purpose of the Study:

  • To investigate the mechanism of action between miR-195-5p and Yes-associated protein 1 (YAP1).
  • To elucidate the role of the miR-195-5p/YAP1 axis in cervical cancer progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure miR-195-5p and YAP1 expression.
  • Cell proliferation, migration, and invasion assays (MTT, wound healing, transwell).
  • Dual luciferase reporter assay, qRT-PCR, and Western blot to confirm YAP1 as a miR-195-5p target.

Main Results:

  • miR-195-5p levels negatively correlate with YAP1 protein, not mRNA.
  • miR-195-5p upregulation inhibited proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in cervical cancer cells.
  • YAP1 was confirmed as a direct target of miR-195-5p, mediating its tumor-suppressive effects.

Conclusions:

  • miR-195-5p functions as a tumor suppressor in cervical cancer.
  • The miR-195-5p/YAP1 pathway presents a potential therapeutic target for cervical cancer.
Keywords:
YAP1cervical cancerepithelial to mesenchymal transitionmiR-195-5p

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