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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Technological advances and computational approaches for alternative splicing analysis in single cells.

Wei Xiong Wen1,2, Adam J Mead1,3, Supat Thongjuea2,3

  • 1MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, UK.

Computational and Structural Biotechnology Journal
|February 27, 2020
PubMed
Summary

Single-cell alternative splicing analysis reveals RNA dynamics missed by bulk methods. This emerging field promises new insights into cellular function and personalized medicine applications.

Keywords:
Alternative splicingIsoformNext-generation sequencingPercent spliced-inSingle-cell transcriptome analysis

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Alternative splicing of RNAs generates diverse protein isoforms crucial for cellular function and identity.
  • Next-generation sequencing, particularly bulk RNA-sequencing, has advanced the study of RNA splicing under various conditions.
  • Single-cell RNA-sequencing has primarily focused on gene-level expression, identifying cell-type-specific signatures.

Purpose of the Study:

  • To review technological advancements in single-cell alternative splicing analysis.
  • To discuss computational strategies for isoform detection and quantification in single cells.
  • To explore current applications and future potential of single-cell alternative splicing in personalized medicine.

Main Methods:

  • Technological advancements in single-cell transcriptomics.
  • Computational approaches for isoform detection and quantification.
  • Review of existing literature and case studies.

Main Results:

  • Single-cell alternative splicing offers a deeper view into transcriptomic dynamics beyond gene-level expression.
  • Emerging technologies and computational tools are enabling more robust single-cell splicing analysis.
  • The field is rapidly advancing, with growing applications in understanding cellular heterogeneity.

Conclusions:

  • Single-cell alternative splicing analysis is a powerful emerging research area.
  • It has the potential to uncover transcriptomic dynamics previously undetectable.
  • Future contributions to personalized medicine are anticipated through detailed cellular analysis.