Related Experiment Video
Updated: Dec 27, 2025

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Draft genome sequence of Actinocorallia populi A251T, an actinomycetes producing polyketides and nonribosomal
Xiaoguang Li1, Zhikuan Wang1, Longzhan Gan1
1Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, College of Biomass Science and Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu, 610065 People's Republic of China.
Abstract:
In this work, we present the draft genome sequence of Actinocorallia populi A251T consisting of 8,253,402 bp with a G + C content of 71.5 mol%. The genome sequence includes 3 5S rRNA genes, 54 tRNA genes, 28 snRNA genes and 19 CRISPRs in 26 contigs. Using the Rapid Annotation using Subsystem Technology, a total of 7766 coding DNA sequences, which were assigned to 436 subsystems, were found in the genome. And 6743 protein-coding sequences with designated functions were assigned to 19 categories in the Cluster of Orthologous Groups database. The genome contains 20 gene clusters responsible for the synthesis of secondary metabolites, including two type I polyketide synthase (PKS) gene clusters, two type III PKS gene clusters, three nonribosomal peptide synthetase (NRPS) gene clusters and two hybrid PKS/NRPS gene clusters. These results indicate that strain A251 has the ability to produce several nonribosomal polypeptides and diverse polyketides, which was verified by liquid chromatography-mass spectrometry analysis of the extraction from fermentation broth. In addition, lactones, indole and many unknown natural products are present in the metabolites. Bioactivity testing revealed anticancer and antidiabetic activities of the crude extract towards the cancer cell line HepG2. In summary, the genomic sequences and bioactive fermentation products demonstrate the potential of strain A251 in biotechnology.
Related Concept Videos
Bacterial Phylum Actinobacteria
Introduction to Actin
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Cytoskeletal Proteins in Bacteria
Bacterial Phylum Planctomycetes

