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Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics.
Youngseop Lee1,2, Byoung-Hoon Kang1,2, Minhee Kang3,4
1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
ACS Applied Materials & Interfaces
|February 27, 2020
Summary
This study introduces nanoplasmonic on-chip PCR for ultrafast molecular diagnostics. The novel technique enables rapid, precise detection of infectious diseases at the point-of-care.
Area of Science:
- Nanotechnology
- Molecular Diagnostics
- Biomedical Engineering
Background:
- Ultrafast polymerase chain reaction (PCR) on chip is crucial for rapid molecular diagnostics.
- Point-of-care testing demands precise and swift infectious disease detection.
Purpose of the Study:
- To develop and demonstrate a nanoplasmonic on-chip PCR system for ultrafast thermal cycling.
- To enable rapid and precise molecular diagnostics for infectious diseases.
Main Methods:
- Fabrication of nanoplasmonic pillar arrays (NPA) with gold nanoislands on glass nanopillars.
- Utilizing enhanced light absorption from white LED excitation for photothermal heating.
- Implementing ultrafast PCR thermal cycling (30 cycles, 98-60 °C in 3 min 30 s).
Main Results:
- Demonstrated rapid DNA amplification of lambda-DNA in 20 cycles.
- Achieved rapid amplification of Middle East respiratory syndrome coronavirus DNA in 30 cycles.
- Validated the technique with conventional PCR volumes (15 μL).
Conclusions:
- Nanoplasmonic on-chip PCR offers a novel approach for rapid precision molecular diagnostics.
- The technology enhances photothermal heating efficiency for accelerated PCR.
- This method presents a significant advancement for point-of-care molecular testing.
