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Cyclin D1 Gene Numerical Imbalances in Laryngeal Squamous Cell Carcinoma: A Tissue Microarray Grid Based Analysis
Efthymios Kyrodimos1, Vasileios Papanikolaou1, Evangelos Tsiambas2,3
11st ENT Dept, Hippocration Hospital, Medical School, University of Athens, Athens, Greece.
Asian Pacific Journal of Cancer Prevention : APJCP
|February 28, 2020
Summary
Cyclin D1 gene amplification in laryngeal squamous cell carcinoma (LSCC) leads to abnormal protein levels and aggressive tumor behavior. This study introduces a novel grid-based CISH method for accurate gene analysis in LSCC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Deregulation of cell cycle proteins like cyclins is common in solid tumors.
- In laryngeal squamous cell carcinoma (LSCC), cyclin D1 overexpression is linked to altered tumor behavior.
- This study investigates the link between cyclin D1 gene copy number and protein expression in LSCC.
Purpose of the Study:
- To determine the correlation between cyclin D1 gene numerical imbalances and protein expression levels in LSCC.
- To assess the association of cyclin D1 deregulation with tumor stage and grade.
Main Methods:
- Fifty primary LSCC tissues were analyzed using tissue microarray (TMA).
- Immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) were employed.
- A novel real-time reference and calibration grid platform was used for CISH analysis.
Main Results:
- Cyclin D1 protein overexpression was found in 44% of cases, and gene amplification in 26%.
- Combined cyclin D1 gene and protein deregulation correlated significantly with malignancy stage (p=0.0014).
- Overall cyclin D1 protein expression strongly correlated with tumor grade (p=0.001).
Conclusions:
- Cyclin D1 gene amplification drives aberrant protein expression in LSCC, correlating with aggressive tumor behavior.
- This study presents the first grid-based CISH analysis for gene numerical imbalances under bright field microscopy.
- The novel CISH method offers accurate screening and mapping across the entire slide area.
