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DUSP9 Suppresses Proliferation and Migration of Clear Cell Renal Cell Carcinoma via the mTOR Pathway

Jing Luo1, Xing Luo1, Xin Liu1

  • 1Department of Urology, Second Affiliated Hospital, Army Medical University, Chongqing, People's Republic of China.

Oncotargets and Therapy
|February 28, 2020
PubMed
Abstract

Insights

Dual specificity phosphatase 9 (DUSP9) acts as a tumor suppressor in clear cell renal cell carcinoma (ccRCC). Lower DUSP9 levels correlate with ccRCC, and its restoration inhibits tumor growth and migration by affecting the mTOR pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Urology

Background:

  • Clear cell renal cell carcinoma (ccRCC) is a prevalent urologic malignancy with incompletely understood carcinogenic mechanisms.
  • Investigating novel molecular players is crucial for understanding ccRCC pathogenesis.

Purpose of the Study:

  • To elucidate the role and mechanism of dual specificity phosphatase 9 (DUSP9) in clear cell renal cell carcinoma.
  • To determine if DUSP9 functions as a tumor suppressor in ccRCC.

Main Methods:

  • Assessed cell proliferation and migration using Cell Counting kit-8, scratch, and Transwell assays.
  • Quantified mRNA and protein expression via qPCR, Western blot, and immunohistochemistry.
  • Utilized a nude mouse xenograft model to evaluate DUSP9's in vivo anti-tumor effects.

Main Results:

  • DUSP9 expression was significantly reduced in ccRCC cell lines and tissues compared to normal controls.
  • DUSP9 suppressed ccRCC cell proliferation and migration in vitro.
  • In vivo studies confirmed DUSP9's inhibition of tumor growth.
  • DUSP9 downregulated mTOR phosphorylation and key pathway proteins (Sox2, c-Myc, HIF-1α) involved in proliferation and migration.

Conclusions:

  • DUSP9 functions as a tumor suppressor in ccRCC.
  • DUSP9 regulates ccRCC cell proliferation and migration through the mTOR signaling pathway.
Keywords:
clear cell renal cell carcinomadual specificity phosphatase 9mTORmigrationproliferation

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