An "OR-AND" logic gate based multifunctional colorimetric sensor for the discrimination of Pb²⁺ and Cd²

Pb²⁺ and Cd²⁺ are the most ubiquitous heavy metal ion pollutants, and they have aroused much attention due to their irreversible and significant damage to human organ. In this work, a new fluorescein-based "OR-AND" logic gate colorimetric probe 3',6'-bis((tert-butyldiphenylsilyl)oxy)-2-(2-((2-hydroxyphenyl)imino)ethylidene)aminspiro[isoindoline-1,9'-xanthen]-3-one (FP) was designed and synthesized via attaching 2-(2-((2-hydroxyphenyl)imino)ethylidene)amino and tert-butyldiphenylsilyl to fluorescein as the specific identification groups. This sensor can rapidly and sensitively discriminate Pb²⁺ and Cd²⁺ by utilizing F^- as an auxiliary reagent. When Pb²⁺ or Cd²⁺ was added into the FP solution, the absorption band at 533 nm increased and the peak at 374 nm decreased, the color changed from colorless to pale-purple, resulting in a ratiometric spectral change. However, adding fluoride ion to the FP solution containing Pb²⁺ or Cd²⁺ resulted in a distinct phenomenon in which the pale purple color fades out to colorless for a Pb²⁺-containing solution and deepen to dark purple for a Cd²⁺-containing solution, which is attributable to the different coordination mechanisms. In aqueous solution, the detection limits of FP can reach 0.42 μM for Pb²⁺ and 0.53 μM for Cd²⁺. The probe exhibited rapid responses for these analytes. Moreover, FP was successfully used to rapidly detect trace amounts of hazardous Pb²⁺ and Cd²⁺ in tap water with good relative recovery and the relative standard deviations (RSD) were 1.8% for Pb²⁺ and 0.3% for Cd²⁺, providing a novel approach for detecting Pb²⁺ and Cd²⁺ in practical application.