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Molecule mechanisms of Ganoderma lucidum treated hepatocellular carcinoma based on the transcriptional profiles and

Chenchen Tang1, Ruolin Zhao2, Hongmei Ni3

  • 1Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|February 29, 2020
PubMed

Insights

Ganoderma lucidum extract (GLE) inhibits tumor growth in mice. Researchers identified key microRNAs and mRNAs involved in this process, suggesting potential biomarkers for evaluating GLE efficacy in cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Ganoderma lucidum (GLE) shows promise in treating cancers like pancreatic cancer and hepatocellular carcinoma.
  • The precise molecular mechanisms of GLE's therapeutic effects remain largely unknown.
  • Understanding these mechanisms is crucial for optimizing cancer treatment strategies.

Purpose of the Study:

  • To investigate the therapeutic efficacy of Ganoderma lucidum extract (GLE) in a mouse model of hepatocellular carcinoma (Hepa1-6).
  • To identify differentially expressed microRNAs (DE-miRNAs) and messenger RNAs (DE-mRNAs) affected by GLE treatment.
  • To elucidate the molecular pathways and potential biomarkers involved in GLE-mediated tumor inhibition.

Main Methods:

  • Hepa1-6 bearing C57 BL/6 mouse model was used to assess GLE's anti-tumor effects.
  • MicroRNA (miRNA) and messenger RNA (mRNA) profiling was performed on tumor tissues.
  • Bioinformatic analyses including network construction (miRNA-target, protein-protein interaction) and qRT-PCR were employed.

Main Results:

  • GLE significantly inhibited tumor growth in the Hepa1-6 mouse model.
  • 25 differentially expressed miRNAs (24 up, 1 down) and 76 differentially expressed mRNAs were identified.
  • Eight hub miRNAs were identified, and five key mRNAs (Cntn1, Irs1, Nfkbia, Rybp, Ywhaz) were found to be downregulated by GLE.
  • Four specific miRNAs were identified as potential regulators of these downregulated mRNAs.

Conclusions:

  • Ganoderma lucidum extract demonstrates significant anti-tumor activity in hepatocellular carcinoma.
  • Specific miRNAs (mmu-mir-23a-5p, -3102-3p, -337-3p, -467a-3p) may play a role in mediating GLE's effects by downregulating key genes.
  • These miRNAs represent potential biomarkers for assessing the efficacy of Ganoderma lucidum in cancer therapy.
Keywords:
Differential expressed geneGanoderma lucidumProtein-protein interaction (PPI)miRNA-target network

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