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BDNF Expression in Cortical GABAergic Interneurons.

Federico José Barreda Tomás1,2, Paul Turko1, Heike Heilmann1

  • 1Institute of Integrative Neuroanatomy, Charité-Universitätsmedizin Berlin, Campus Mitte, 10117 Berlin, Germany.

International Journal of Molecular Sciences
|February 29, 2020
PubMed
Summary
This summary is machine-generated.

Brain-derived neurotrophic factor (BDNF) is synthesized by both principal cells and interneurons in the mouse cortex. This study confirms BDNF production in both glutamatergic and GABAergic neurons, expanding our understanding of neurotrophin distribution.

Keywords:
BDNFFACSGABAergic interneuronsRT-qPCRhippocampuslaser capture microdissectionneocortex

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Brain-derived neurotrophic factor (BDNF) is crucial for neuronal survival and function.
  • BDNF is primarily known to be produced by glutamatergic principal cells in the cortex.
  • The synthesis of BDNF by GABAergic interneurons remains largely uncharacterized.

Purpose of the Study:

  • To investigate the cellular localization and synthesis of BDNF in both glutamatergic and GABAergic neurons.
  • To determine if GABAergic interneurons contribute to BDNF production in the mouse cortex.
  • To elucidate the cellular distribution of proBDNF and its RNA in different neuronal populations.

Main Methods:

  • Immunocytochemical labeling to detect proBDNF and mature BDNF (mBDNF) proteins.
  • Reverse transcription quantitative PCR (RT-qPCR) to quantify BDNF RNA.
  • Laser capture microdissection (LCM) and fluorescence-activated cell sorting (FACS) for cell-specific analysis.

Main Results:

  • Immunofluorescence confirmed the presence of both proBDNF and mBDNF in both glutamatergic and GABAergic neurons.
  • ProBDNF exhibited a perinuclear distribution, co-localizing with the rough endoplasmic reticulum.
  • RT-qPCR analysis revealed abundant BDNF transcripts in both neuronal types from the cortex and hippocampus.

Conclusions:

  • BDNF is synthesized by both principal glutamatergic neurons and GABAergic interneurons in the mouse cortex.
  • This finding expands the known cellular sources of BDNF, suggesting broader roles in cortical circuitry.
  • The study provides compelling evidence for dual synthesis of BDNF by major neuronal populations in the central nervous system.