Related Experiment Video

Updated: Dec 27, 2025

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Published on: November 1, 2021

2.7K

Direct interaction between CEP85 and STIL mediates PLK4-driven directed cell migration

Yi Liu1,2, Jaeyoun Kim1, Reuben Philip1,2

  • 1Lunenfeld-Tanenbaum Research Institute, University of Toronto, 600 University Avenue, Toronto M5G 1X5, Canada.

Journal of Cell Science
|February 29, 2020
PubMed

Insights

CEP85 and STIL are crucial for cancer cell migration, working with PLK4 to promote movement. Their interaction with PLK4 is essential for directional motility and invasion.

Area of Science:

  • Cell Biology
  • Cancer Research
  • Molecular Oncology

Background:

  • Polo-like kinase 4 (PLK4) is overexpressed in many cancers and has an emerging role in cancer cell migration and invasion.
  • The molecular mechanisms underlying PLK4's role in cell motility are not fully understood.
  • Previous research established the CEP85-STIL binding interface's necessity for PLK4 activation and centriole duplication.

Purpose of the Study:

  • To investigate the role of CEP85 and STIL in directional cancer cell migration.
  • To elucidate the molecular mechanisms by which CEP85, STIL, and PLK4 interact to regulate cell motility.

Main Methods:

  • Mutational and functional analyses of CEP85, STIL, and PLK4 interactions.
  • Investigating the recruitment of CEP85 and STIL to the leading edge of migrating cells.
  • Assessing the impact of CEP85 and STIL downregulation on ARP2 phosphorylation and actin cytoskeleton organization.

Main Results:

  • CEP85 and STIL are essential for directional cancer cell migration.
  • The interaction between CEP85, STIL, and PLK4 is critical for effective cell motility.
  • PLK4 recruits CEP85 and STIL to the leading edge, promoting cell protrusion.
  • Downregulation of CEP85 and STIL reduces ARP2 phosphorylation and actin reorganization, impairing migration.

Conclusions:

  • The CEP85-STIL complex plays a significant role in modulating PLK4-driven cancer cell migration.
  • These findings provide molecular insights into the CEP85-STIL complex's function in cancer cell motility.
  • Targeting the CEP85-STIL-PLK4 axis could offer new therapeutic strategies for cancer treatment.
Keywords:
ActinCEP85Cell motilityCentrioleCentrosomePLK4STIL

More Related Videos

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration10:43

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

7.0K
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion09:23

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Published on: April 2, 2020

6.8K

Related Concept Videos

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.3K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.4K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.0K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.3K
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.3K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.4K