Related Experiment Video

Updated: Dec 27, 2025

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
09:23

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Published on: April 2, 2020

6.8K

Circ-IGF1R inhibits cell invasion and migration in non-small cell lung cancer

Zhanyu Xu1, Weiwei Xiang1, Wenjie Chen1

  • 1Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Thoracic Cancer
|February 29, 2020
PubMed
Abstract

Insights

Circular RNA (circ-IGF1R) is downregulated in lung cancer, inhibiting tumor invasion and migration. This study elucidates its mechanism via the circ-IGF1R-miR-1270-VANGL2 network, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are emerging biomarkers for tumor invasion and migration.
  • The specific role of hsa_circ_0005035 (circ-IGF1R) in non-small cell lung cancer (NSCLC) requires elucidation.

Purpose of the Study:

  • To investigate the mechanism of action of circ-IGF1R in lung cancer.
  • To determine the association between circ-IGF1R expression and NSCLC progression.

Main Methods:

  • Screening of circ-IGF1R from the Gene Expression Omnibus database (GSE104854).
  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to assess circ-IGF1R expression in NSCLC cell lines and tissues.
  • Wound-healing and Transwell assays to evaluate the functional role of circ-IGF1R.
  • RNA-sequencing (RNA-seq) to explore the circ-IGF1R-mediated transcriptome changes.

Main Results:

  • circ-IGF1R expression was significantly lower in lung cancer tissues and cell lines compared to normal controls (P < 0.0001).
  • Reduced circ-IGF1R levels correlated with advanced tumor stage (T2/T3/T4) and lymph node metastasis (N1/N2/N3) (P < 0.05).
  • Overexpression of circ-IGF1R suppressed lung cancer cell invasion and migration.

Conclusions:

  • circ-IGF1R acts as a tumor suppressor in lung cancer.
  • The inhibitory effect on invasion and migration is potentially mediated by the circ-IGF1R-miR-1270-VANGL2 axis.
  • circ-IGF1R represents a promising molecular marker and therapeutic target for NSCLC.
Keywords:
Circ-IGF1RNSCLCcircular RNAinvasionmigration

More Related Videos

Author Spotlight: Assessing the Potential of Circulating Tumor Cells in Leptomeningeal Disease Research06:25

Author Spotlight: Assessing the Potential of Circulating Tumor Cells in Leptomeningeal Disease Research

Published on: March 29, 2024

1.4K
A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion06:36

A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion

Published on: April 24, 2015

11.3K

Related Concept Videos

Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.1K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.6K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.1K
Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
4.8K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.5K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.4K