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Related Concept Videos

Mass Analyzers: Overview01:13

Mass Analyzers: Overview

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
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Preparative Mass Spectrometry Using a Rotating-Wall Mass Analyzer.

Pei Su1, Hang Hu1, Daisy Unsihuay1

  • 1Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA.

Angewandte Chemie (International Ed. in English)
|February 29, 2020
PubMed
Summary
This summary is machine-generated.

A new technique precisely controls the high-throughput deposition of ions, from nanocrystals to proteins, onto surfaces. This method advances materials science and energy technology by enabling detailed structural characterization and property screening.

Keywords:
electron microscopygold nanoclustershigh throughputmass spectrometrysurface preparation

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Area of Science:

  • Materials Science
  • Energy Technology
  • Surface Science

Background:

  • Functional interface design is crucial for materials science and energy research.
  • Precisely controlled preparation of interfaces with diverse species is challenging.
  • Existing methods lack the throughput and control for complex polyatomic species.

Purpose of the Study:

  • To introduce a broadly applicable technique for high-throughput interface preparation.
  • To enable precise control over the deposition of polyatomic species, including ions, nanocrystals, and protein complexes.
  • To facilitate advanced structural characterization and property screening of engineered interfaces.

Main Methods:

  • Utilized a rotating-wall mass analyzer for mass-dispersive ion deposition.
  • Employed low voltages for ion separation with a theoretically unlimited mass range.
  • Demonstrated efficient deposition of gold nanocrystal ions (Au144(SC4H9)60, 33.7 kDa).

Main Results:

  • Achieved precisely controlled, high-throughput deposition of well-defined interfaces.
  • Successfully deposited singly charged gold nanocrystal ions.
  • Showcased the capability for mass-selected ion deposition from multicomponent mixtures.

Conclusions:

  • The developed technique offers a versatile platform for preparing functional interfaces.
  • Enables advanced structural characterization of nanocrystals and their assemblies via transmission electron microscopy.
  • Supports controlled preparation of surface gradients and rapid molecular property screening.