Related Experiment Video
Updated: Dec 27, 2025
12:22
The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
Published on: January 22, 2013
34.0K
Quantification of Biomarker Proteins Using Reverse-Phase Protein Arrays.
1National Cancer Center Research Institute, Tokyo, 104-0045, Japan.
Proteomics. Clinical Applications
|February 29, 2020
Summary
Antibody-based proteomics, like reverse-phase protein array (RPPA), is crucial for biomarker discovery. A new study compares RPPA and immunohistochemistry for quantifying lymphoma biomarkers, enhancing diagnostic accuracy.
Area of Science:
- Biotechnology and Proteomics
- Clinical Diagnostics
- Oncology
Background:
- Antibody-based proteomics is emerging as a key technology for biomarker and therapeutic target discovery and validation.
- Reverse-phase protein array (RPPA) is a sensitive antibody-based method for quantifying post-translationally modified proteins in small samples.
- RPPA's multiplexing capability and expandability make it suitable for clinical proteomics and biomarker research.
Purpose of the Study:
- To compare the efficacy of Reverse-Phase Protein Array (RPPA) with immunohistochemistry (IHC) for quantifying biomarker proteins.
- To evaluate the utility of these methods in subtyping diffuse large B-cell lymphoma (DLBCL).
- To assess the potential of multiplex biomarker quantification for improving diagnostic accuracy in heterogeneous diseases like DLBCL.
Main Methods:
- Utilized Reverse-Phase Protein Array (RPPA) to quantify protein expression.
- Employed immunohistochemistry (IHC) as a comparative method.
- Focused on seven specific biomarker proteins critical for DLBCL subtyping.
Main Results:
- The study presents the first direct comparison of RPPA and IHC for quantifying multiple DLBCL biomarker proteins.
- RPPA demonstrated high sensitivity and required minimal sample amounts, suitable for clinical applications.
- The combined analysis of multiple biomarkers showed potential for increased diagnostic accuracy.
Conclusions:
- RPPA is a valuable tool for clinical proteomics, offering sensitive and efficient quantification of protein biomarkers.
- Comparing RPPA and IHC provides insights into optimal methods for biomarker quantification in lymphoma.
- Multiplex biomarker analysis using RPPA can significantly enhance the precise classification of heterogeneous diseases like DLBCL.
Related Concept Videos
02:57Real Time RT-PCR
64.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
64.2K
01:33Proteomics
9.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.2K