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Profiling the urinary microbiome in men with calcium-based kidney stones
Jing Xie1, Jian-Sheng Huang1, Xiang-Jiang Huang1
1Department of Urology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, 518020, China.
BMC Microbiology
|March 1, 2020
Summary
The urinary microbiome of male kidney stone patients shows reduced diversity and altered composition compared to healthy individuals. This dysbiosis may link urinary bacteria to calcium-based kidney stone formation.
Area of Science:
- Microbiology
- Urology
- Genomics
Background:
- Recent molecular methods challenge the long-held belief that urine is sterile in healthy individuals.
- Growing evidence links imbalances in the urinary microbiota (dysbiosis) to various urological conditions.
- The urinary microbiome's role in kidney stone formation is an emerging area of research.
Purpose of the Study:
- To investigate the urinary microbiome composition in male patients diagnosed with calcium-based kidney stones.
- To compare the urinary microbiome of kidney stone patients with that of healthy controls.
- To explore potential associations between urinary microbiota and calcium-based kidney stones.
Main Methods:
- Analysis of urinary microbiome diversity using Shannon and Simpson indices.
- Comparison of urinary microbiota structure between patient and control groups using ANOSIM.
- Identification of differentially represented bacteria and enriched functional pathways in urine samples.
Main Results:
- Kidney stone patients exhibited significantly lower urinary microbiota diversity compared to healthy controls (P < 0.001).
- Distinct urinary microbiota structures were observed between kidney stone patients and healthy individuals (ANOSIM, R = 0.11, P < 0.001).
- Specific bacteria associated with inflammation (e.g., Acinetobacter) and certain functional pathways were enriched in kidney stone patients' urine.
Conclusions:
- A significant dysbiosis of the urinary microbiota was identified in male patients with calcium-based kidney stones.
- These findings suggest a potential role for urinary microbiota alterations in the pathogenesis of calcium-based kidney stones.
- Urinary microbiota composition was consistent between bladder and renal pelvis urine in kidney stone patients.
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