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MircoRNA-129-5p suppresses the development of glioma by targeting HOXC10

Jin Liu1, Chao Cheng1, Jiantong Jiao1

  • 1Department of Neurosurgery, Wuxi People's Hospital of Nanjing Medical University, Wuxi, 214023, Jiangsu, China.

Pathology, Research and Practice
|March 1, 2020
PubMed
Abstract

Insights

MicroRNA-129-5p (miR-129-5p) inhibits glioma progression by targeting HOXC10. This finding suggests miR-129-5p as a potential diagnostic marker and therapeutic target for glioma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-129-5p (miR-129-5p) is implicated in various cancers but its role in gliomas requires further investigation.
  • The precise molecular mechanisms underlying miR-129-5p's function in glioma remain unclear.

Purpose of the Study:

  • To elucidate the role and molecular mechanism of miR-129-5p in glioma.
  • To investigate the potential of miR-129-5p as a diagnostic marker and therapeutic target for glioma.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to assess miR-129-5p and HOXC10 expression.
  • In vitro assays (CCK-8, EdU, clone formation) to evaluate cell proliferation, migration, and invasion.
  • Luciferase assays to confirm direct targeting of HOXC10 by miR-129-5p.
  • In vivo tumor xenograft model to assess the impact of miR-129-5p on glioma growth.

Main Results:

  • miR-129-5p expression was significantly downregulated in glioma tissues and cell lines.
  • Overexpression of miR-129-5p suppressed glioma cell proliferation, migration, and invasion.
  • miR-129-5p directly targets HOXC10, which is upregulated in gliomas.
  • Knockdown of HOXC10 inhibited glioma cell proliferation, migration, and invasion.

Conclusions:

  • miR-129-5p exerts tumor-suppressive effects in glioma by downregulating HOXC10.
  • miR-129-5p represents a promising diagnostic biomarker and therapeutic strategy for glioma.
Keywords:
GliomaHOXC10ProliferationmiR-129-5p

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