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Related Experiment Video

Updated: Dec 27, 2025

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
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Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo.

Dong Han1,2, Joseph C Wu3,4,5

  • 1Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 1, 2020
PubMed
Summary

Bioluminescence imaging allows noninvasive, real-time tracking of engrafted stem cells in vivo. This method monitors cell survival and fate after transplantation without needing to sacrifice animals.

Keywords:
BioluminescenceCell trackingLuciferaseMolecular imaging

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Area of Science:

  • Biomedical imaging
  • Cell biology
  • Stem cell research

Background:

  • Bioluminescence imaging (BLI) offers noninvasive, real-time detection of biological processes.
  • Tracking engrafted cells in vivo is crucial for understanding cell fate after transplantation.
  • Current methods may require animal sacrifice, limiting dynamic monitoring.

Purpose of the Study:

  • To describe a protocol for in vivo tracking of luciferase-labeled stem cells.
  • To demonstrate the utility of bioluminescence imaging for monitoring cell survival and retention.
  • To provide a noninvasive method for assessing cell fate after transplantation.

Main Methods:

  • Utilizing luciferase-labeled stem cells.
  • Employing bioluminescence imaging (BLI) for photon detection.
  • Using an IVIS spectrum imaging system for data acquisition.

Main Results:

  • Successfully imaged and tracked luciferase-labeled stem cells in vivo.
  • Demonstrated the ability to monitor engrafted cell survival and retention dynamically.
  • Quantified photons released from luciferin oxidation catalyzed by luciferase.

Conclusions:

  • Bioluminescence imaging is an effective tool for noninvasive, real-time monitoring of stem cell fate.
  • This protocol facilitates dynamic tracking of engrafted cells without animal sacrifice.
  • BLI provides valuable insights into cell survival and retention post-transplantation.