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Different Polymorphisms of Vascular Endothelial Growth Factor Gene in Patients with Pre-Eclampsia among The Iranian
Rana Niktalab1, Zeinab Piravar1, Roudabeh Behzadi1
1Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Insights
Vascular endothelial growth factor (VEGF) gene polymorphism rs10434 is associated with pre-eclampsia (PE) incidence in Iranian women. The recessive allele (G) for rs10434 was significantly more frequent in women with PE.
Area of Science:
- Genetics
- Obstetrics
- Molecular Biology
Background:
- Pre-eclampsia (PE) is a major cause of maternal and neonatal mortality worldwide.
- PE is characterized by hypertension and organ damage, often affecting the liver and kidneys.
- Genetic factors, particularly mutations in the vascular endothelial growth factor (VEGF) gene, are implicated in PE development.
Purpose of the Study:
- To investigate the association between specific VEGF gene polymorphisms (rs922583280, rs3025040, and rs10434) and the incidence of PE.
- To analyze the relationship of these VEGF polymorphisms in the Iranian female population.
Main Methods:
- A case-control study involving 100 pregnant women diagnosed with PE and 50 healthy pregnant women.
- Sanger sequencing was employed to determine the genotypes of the specified VEGF polymorphisms.
Main Results:
- No significant difference in allele frequencies for rs922583280 and rs3025040 between PE cases and controls.
- A significantly higher frequency of the recessive allele (G) for rs10434 polymorphism was observed in the PE group (24%) compared to the control group (12%).
- Allele A and AA genotype frequencies were higher in controls, while AG genotype was more prevalent in the PE group for rs10434.
Conclusions:
- The VEGF gene polymorphism rs10434 shows a significant association with pre-eclampsia incidence.
- This specific polymorphism may play a role in the development of PE among Iranian women.
Background:
Pre-eclampsia (PE) is a pregnancy complication and one of the leading causes of maternal and neonatal morbidity and mortality in the world. PE is characterized by high blood pressure and signs of damage to the other organs, most often the liver and kidneys. Given the importance of mutation in the vascular endothelial growth factor (VEGF) gene and its correlation with the incidence of PE, the relationship of VEGF encoding gene polymorphisms rs922583280, rs3025040 and rs10434 with the incidence of PE in the population of Iranian women was studied, in this research.
Materials And Methods:
In this case-control study, 100 pregnant women with PE diagnosis and 50 healthy pregnant women were evaluated using Sanger sequencing method to determine genotypes rs922583280, rs3025040 and rs10434.
Results:
There was no significant difference in the allele frequency of rs922583280 and rs3025040 polymorphisms between case and control groups (P>0.05), while frequency of the recessive allele (G) for rs10434 polymorphism was significantly higher in the case group compared to the control group (P=0.014, case=24%, control=12%). Frequency of the allele A in the control group was higher than the patient group (case=76%, control=88%). Frequency of AG genotype in the patient group was also higher than the control group. In addition, frequency of AA genotype in the control group was higher than the patient group (case=57%, control=78).
Conclusion:
The results of this study demonstrated a significant difference between patient and control groups for the VEGF coding gene polymorphism rs10434 and it can affect the incidence of PE among Iranian women.
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