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Differential effects of adrenocorticotropin(1-24) on [3H]2-deoxy-D-glucose uptake in cultured cells derived from
T Bistritzer1, P A Levin, L M Roeder
1Department of Pediatrics, University of Maryland School of Medicine, Baltimore 21201.
Abstract:
The effect of adrenocorticotropin(1-24) (ACTH(1-24)) on the uptake of [3H]2-deoxy-D-glucose ([3H]2-DG) was compared in cell cultures derived from two regions (hypothalamus, and extrahypothalamic forebrain) of fetal rat brain. Under control conditions, [3H]2-DG uptake was similar in extrahypothalamic (10.9 +/- 1.1 nmol/mg protein/5 min) and hypothalamic (11.9 +/- 1.3) cells. No significant effect of ACTH (1-24) (10(-7) to 10(-5) M) was found on uptake of [3H]2-DG in extrahypothalamic cells. In contrast, in hypothalamic cells, a potent stimulatory effect (P less than 0.0001) up to 174% over the control value of [3H]2-DG uptake was produced by these concentrations of ACTH(1-24). This study suggests that ACTH may be a stimulator of brain glucose uptake, and that this effect varies in different brain regions.