Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fast Fourier Transform01:10

Fast Fourier Transform

800
The Fast Fourier Transform (FFT) is a computational algorithm designed to compute the Discrete Fourier Transform (DFT) efficiently. By breaking down the calculations into smaller, manageable sections, the FFT significantly reduces the computational complexity involved. Direct computation of an N-point DFT requires N2 complex multiplications, whereas the FFT algorithm needs only (N/2)log⁡2N multiplications, offering a much faster performance.
The computational efficiency of the FFT becomes...
800
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

559
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
559
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

669
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
669
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.8K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.8K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.3K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.3K
Discrete Fourier Transform01:15

Discrete Fourier Transform

761
The Discrete Fourier Transform (DFT) is a fundamental tool in signal processing, extending the discrete-time Fourier transform by evaluating discrete signals at uniformly spaced frequency intervals. This transformation converts a finite sequence of time-domain samples into frequency components, each representing complex sinusoids ordered by frequency. The DFT translates these sequences into the frequency domain, effectively indicating the magnitude and phase of each frequency component present...
761

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intestinal Absorption and Anti-Inflammatory Effects of a Low-Molecular-Weight α-Glucan from <i>Flammulina filiformis</i>.

Foods (Basel, Switzerland)·2026
Same author

Size of Biomolecular Condensates Dictates Fate in Liquid-Solid Phase Transitions through Amorphous-Amyloid Competition.

Journal of the American Chemical Society·2026
Same author

Lipids Contribute to Heterochromatin Condensation Revealed by Quantitative Raman-Brillouin Microscopy.

JACS Au·2026
Same author

Micranthin B alleviates metabolic dysfunction-associated steatohepatitis by targeting G3BP1 to improve stress granule-mediated endoplasmic reticulum stress.

Chinese journal of natural medicines·2026
Same author

Hydrogen Bond-Regulated Rigid Bis-pyridine-Flanked Thiophene Derivatives for Organic Field-Effect Transistors.

The Journal of organic chemistry·2026
Same author

Histone lactylation increases CXCL1 expression for neutrophil infiltration and immune escape in pancreatic cancer.

Nature communications·2026

Related Experiment Video

Updated: Dec 27, 2025

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

7.5K

Practical Computation of FFT-based Generalized Two-dimensional Correlation Spectroscopy.

Mengmeng He1, Daisuke Miyata2, Takakazu Nakabayashi2

  • 1Graduate School of Science, Tohoku University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|March 3, 2020
PubMed
Summary

A new computational method for generalized two-dimensional (2D) correlation spectroscopy using fast Fourier transformation (FFT) was developed. This versatile technique accurately analyzes various waveforms, proving effective regardless of data point variations.

Keywords:
CorrelationFFTFTcomputationsinusoidalspectroscopy

More Related Videos

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.9K
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.3K

Related Experiment Videos

Last Updated: Dec 27, 2025

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

7.5K
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.9K
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.3K

Area of Science:

  • Spectroscopy
  • Computational Chemistry
  • Data Analysis

Background:

  • Two-dimensional (2D) correlation spectroscopy is a powerful technique for analyzing complex spectral data.
  • Fast Fourier Transformation (FFT) is a widely used algorithm for spectral analysis.
  • Existing methods for 2D correlation spectroscopy can be computationally intensive and sensitive to data parameters.

Purpose of the Study:

  • To develop a practical and computationally efficient method for generalized 2D correlation spectroscopy.
  • To validate the robustness and versatility of the proposed FFT-based method.
  • To provide a reliable tool for analyzing diverse waveform data in spectroscopic studies.

Main Methods:

  • Implementation of a generalized 2D correlation spectroscopy algorithm utilizing fast Fourier transformation (FFT).
  • Testing the computational method with simple sinusoidal waveforms to assess its performance.
  • Evaluating the method's invariance to changes in the number of data points in time-domain profiles.

Main Results:

  • The FFT-based generalized 2D correlation spectroscopy computation was successfully implemented.
  • The method demonstrated effectiveness and accuracy when tested with sinusoidal data.
  • The computation showed invariance to variations in the number of data points, confirming its robustness.

Conclusions:

  • The described FFT-based generalized 2D correlation spectroscopy offers a practical and versatile computational approach.
  • This method is suitable for analyzing various types of waveforms in spectroscopic applications.
  • The technique's invariance to data point numbers enhances its reliability and applicability.