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Hemoglobinometry: evaluation of a new method with a stable primary standard.
1Red Cross Blood Transfusion Service, Sydney.
Pathology
|April 1, 1988
Summary
A new hemoglobinometry method using chlorohemin as a stable standard offers advantages over the cyanmethemoglobin technique. This method provides accurate hemoglobin measurements with a long-lasting, reliable standard.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
Background:
- The cyanmethemoglobin method is a standard for hemoglobin measurement but has limitations.
- A stable primary standard for hemoglobinometry is needed.
Purpose of the Study:
- To evaluate a new hemoglobinometry method using purified chlorohemin as a stable standard.
- To compare the new method with the cyanmethemoglobin technique.
Main Methods:
- Absorption spectra of blood samples and chlorohemin were analyzed in an alkaline-detergent solution.
- Stability of the reagent mixture and chlorohemin standard was assessed under various storage conditions.
- The new method was correlated with the cyanmethemoglobin technique.
Main Results:
- Chlorohemin and blood samples exhibited identical absorption spectra with a peak at 575 nm.
- The reagent and chlorohemin standard remained stable for at least 250 days.
- The new method showed close correlation with the cyanmethemoglobin technique, offering advantages such as a stable standard and improved conversion.
Conclusions:
- The newly described hemoglobinometry method using chlorohemin offers significant advantages over the cyanmethemoglobin method.
- This method provides a stable primary standard with a long shelf-life for accurate hemoglobin measurements.
