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Hemoglobinometry: evaluation of a new method with a stable primary standard.

B R Wylie¹, V A Lovric

¹Red Cross Blood Transfusion Service, Sydney.

|April 1, 1988

Summary

A new hemoglobinometry method using chlorohemin as a stable standard offers advantages over the cyanmethemoglobin technique. This method provides accurate hemoglobin measurements with a long-lasting, reliable standard.

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Area of Science:

Clinical Chemistry
Analytical Chemistry

Background:

The cyanmethemoglobin method is a standard for hemoglobin measurement but has limitations.
A stable primary standard for hemoglobinometry is needed.

Purpose of the Study:

To evaluate a new hemoglobinometry method using purified chlorohemin as a stable standard.
To compare the new method with the cyanmethemoglobin technique.

Main Methods:

Absorption spectra of blood samples and chlorohemin were analyzed in an alkaline-detergent solution.
Stability of the reagent mixture and chlorohemin standard was assessed under various storage conditions.
The new method was correlated with the cyanmethemoglobin technique.

Main Results:

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Chlorohemin and blood samples exhibited identical absorption spectra with a peak at 575 nm.

The reagent and chlorohemin standard remained stable for at least 250 days.

The new method showed close correlation with the cyanmethemoglobin technique, offering advantages such as a stable standard and improved conversion.

Conclusions:

The newly described hemoglobinometry method using chlorohemin offers significant advantages over the cyanmethemoglobin method.
This method provides a stable primary standard with a long shelf-life for accurate hemoglobin measurements.