Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

925
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
925
Photoelectric Effect02:26

Photoelectric Effect

38.4K
When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...
38.4K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.3K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.3K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

6.4K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
6.4K
Flame Photometry: Overview01:02

Flame Photometry: Overview

1.3K
Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
1.3K
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

3.3K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Nondestructive Raman Spectral Method for Temporal Tracking of Articular Cartilage Maturation.

Tissue engineering. Part A·2026
Same author

Multi-omics analysis of genetic drivers linking aortic stenosis and left ventricular diastolic dysfunction in heart failure.

BioData mining·2026
Same author

Traumatic Coronary Artery Dissection After a Fall From a Roof.

JACC. Case reports·2026
Same author

A Woman's Heart: The Microvascular Disease Dilemma.

The Canadian journal of cardiology·2026
Same author

Arbuscular Mycorrhizal Fungi and Leonardite alter fatty acid and amino acid biosynthesis in sunflower grains.

Plant molecular biology·2026
Same author

Synergistic application of biochar and mercury-resistant Bacillus cereus enhances phytoremediation efficiency and stress tolerance in sorghum (Sorghum bicolor L.) grown in mercury-contaminated soil.

Biodegradation·2026

Related Experiment Video

Updated: Dec 27, 2025

Fabrication and Testing of Photonic Thermometers
08:44

Fabrication and Testing of Photonic Thermometers

Published on: October 24, 2018

6.2K

A photonic pH sensor based on photothermal spectroscopy.

Matthew R Hartings1, Nathan J Castro2,3, Kathryn Gill2

  • 1Department of Chemistry, American University, Washington, DC, USA.

Sensors and Actuators. B, Chemical
|March 3, 2020
PubMed
Summary

A new fiber optic pH sensor uses anthocyanins and fiber Bragg gratings (FBG) to measure pH in biological samples. This innovative platform shows promise for advanced bio-industrial and tissue engineering applications.

More Related Videos

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.9K
Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution
06:42

Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution

Published on: May 9, 2025

974

Related Experiment Videos

Last Updated: Dec 27, 2025

Fabrication and Testing of Photonic Thermometers
08:44

Fabrication and Testing of Photonic Thermometers

Published on: October 24, 2018

6.2K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.9K
Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution
06:42

Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution

Published on: May 9, 2025

974

Area of Science:

  • Biomedical Engineering
  • Optical Sensing Technologies
  • Analytical Chemistry

Background:

  • Accurate pH determination is crucial for bio-industrial processing, tissue engineering, and intracellular measurements.
  • Traditional pH probes face limitations in specialized environments.
  • Development of novel pH sensing platforms is essential for technological advancement.

Purpose of the Study:

  • To introduce a novel fiber optic-based platform for pH measurement.
  • To utilize anthocyanins as pH-sensitive chromophores and fiber Bragg gratings (FBG) for signal transduction.
  • To assess the platform's viability for biological sample analysis.

Main Methods:

  • A fiber optic platform was developed, incorporating fiber Bragg gratings (FBG) sensitive to temperature changes.
  • Anthocyanins were used as pH-sensitive chromophores, correlating light absorption to heat release.
  • The platform was tested by coating optical fibers with anthocyanin-loaded polyethylene glycol diacrylate (PEG-DA) and measuring FBG signal shifts across a pH range of 2.5 to 10.

Main Results:

  • The platform demonstrated a correlation between anthocyanin light absorption, heat release, and FBG signal shifts across a pH range of 2.5 to 10.
  • Coating the fiber with anthocyanin-loaded PEG-DA enhanced the sensor's signal magnitude compared to solution-based measurements.
  • The FBG peak redshifted with increasing temperature, indicating successful heat detection.

Conclusions:

  • The developed fiber optic platform is a viable tool for assessing pH in biological samples.
  • Incorporating anthocyanins within a PEG-DA matrix improves sensor performance.
  • Further optimization of this platform can lead to advanced pH sensing capabilities for various applications.