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Updated: Dec 27, 2025

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Published on: June 9, 2019
Metabolism in the tumor microenvironment: insights from single-cell analysis
Zhengtao Xiao1, Jason W Locasale2, Ziwei Dai1
1Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC, USA.
Abstract:
The metabolism of both cancer and immune cells in the tumor microenvironment (TME) is poorly understood since most studies have focused on analysis in bulk samples and ex vivo cell culture models. Our recent analyses of single-cell RNA sequencing data suggest that the metabolic features of single cells within TME differ greatly from those of the bulk measurements. Here, we discuss some key findings about metabolism in cancer and immune cells and discuss possible relevance to immunotherapy.
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