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Related Experiment Video

Updated: Dec 27, 2025

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
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Dynamic Alternative Splicing During Mouse Preimplantation Embryo Development.

Yongqiang Xing1,2, Wuritu Yang3, Guoqing Liu1,2

  • 1School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, China.

Frontiers in Bioengineering and Biotechnology
|March 3, 2020
PubMed
Summary
This summary is machine-generated.

This study reveals dynamic alternative splicing (AS) patterns during early mouse development. Key splicing factors are regulated by AS, with zygotic AS activation (ZASA) coinciding with zygotic genome activation (ZGA).

Keywords:
alternative splicinggene expressionpreimplantation developmentsplicing factorszygotic gene activation

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Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Alternative pre-mRNA splicing (AS) mechanisms during early embryogenesis remain poorly understood.
  • Dynamic changes in AS are crucial for regulating gene expression during preimplantation development.

Purpose of the Study:

  • To investigate the dynamic landscape of alternative splicing during mouse preimplantation development.
  • To identify key splicing factors and regulatory networks governing early embryogenesis.

Main Methods:

  • Bioinformatics analysis of single-cell RNA sequencing (scRNA-seq) data from time-course preimplantation development.
  • Detection and analysis of differentially expressed genes and alternative splicing events.
  • Establishment of an atlas for AS and differential AS.

Main Results:

  • Numerous novel differentially expressed genes and alternative splicing events were identified across developmental stages.
  • Differentially alternatively spliced genes (DASGs) were found to be critical splicing factors (SFs).
  • A regulatory cascade of AS was observed, where SFs are regulated by differential AS of their own transcripts. 212 isoform switches (ISs) were detected.
  • Zygotic AS activation (ZASA) was found to be synchronized with zygotic genome activation (ZGA), indicating AS is coupled with transcription.

Conclusions:

  • The study provides a comprehensive atlas of AS during preimplantation development.
  • Key SFs and their regulatory cascades play critical roles in early embryogenesis.
  • The coupling of ZASA with ZGA highlights the importance of AS regulation in early development.