Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

11.5K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.5K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.3K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.3K
RNA Splicing01:32

RNA Splicing

60.1K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.1K
RNA Structure01:23

RNA Structure

78.5K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.5K
RNA Structure01:19

RNA Structure

6.7K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.7K
Leaky Scanning02:28

Leaky Scanning

5.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Muscleblind-like proteins dimerize by forming disulfide bonds to regulate alternative splicing and pathogenic RNA foci formation.

Nucleic acids research·2026
Same author

Convergent effects of neurodevelopmental disorder-associated variants at mitochondria.

bioRxiv : the preprint server for biology·2026
Same author

Muscleblind-like proteins dimerize by forming disulfide bonds to regulate alternative splicing and pathogenic RNA foci formation.

bioRxiv : the preprint server for biology·2026
Same author

Protocol for identifying surface membrane proteins and their associated proteome from mouse cortical neuron cultures by in situ biotinylation.

STAR protocols·2026
Same author

Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes.

bioRxiv : the preprint server for biology·2026
Same author

FMRP-dependent translational control negatively regulates adapter protein complex 2-mediated endocytosis.

iScience·2025
Same journal

Mesenchymal stem cells-derived extracellular vesicles as a novel drug delivery carrier: engineering strategies and clinical safety estimation.

Frontiers in molecular biosciences·2026
Same journal

Preparation and analysis of tobacco glycosides, and the relationship between glycoside aglycones and pyrolysis products: a review.

Frontiers in molecular biosciences·2026
Same journal

Peritoneal metastasis in pancreatic cancer: molecular mechanisms, microenvironmental remodeling, and emerging intraperitoneal interventions.

Frontiers in molecular biosciences·2026
Same journal

Insights from LC-MS-based cerebrospinal fluid metabolomics in tuberculous meningitis.

Frontiers in molecular biosciences·2026
Same journal

Emerging roles of Notch signaling in the tumor microenvironment of digestive system cancers.

Frontiers in molecular biosciences·2026
Same journal

Adenosine metabolism as an endogenous protective mechanism in response to upstream ischemic injury.

Frontiers in molecular biosciences·2026
See all related articles

Related Experiment Video

Updated: Dec 27, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.7K

FUS Recognizes G Quadruplex Structures Within Neuronal mRNAs.

Joshua A Imperatore1, Damian S McAninch1, Arielle N Valdez-Sinon2

  • 1Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, PA, United States.

Frontiers in Molecular Biosciences
|March 3, 2020
PubMed
Summary
This summary is machine-generated.

Fused in sarcoma (FUS) protein binds to specific RNA structures in neuronal mRNAs, suggesting a role in synaptic function and potentially collaborating with FMRP to regulate translation.

Keywords:
ALSFTDFUSG quadruplex RNARGG boxmRNA

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.1K

Related Experiment Videos

Last Updated: Dec 27, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.7K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.1K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • RNA Biology

Background:

  • Fused in sarcoma (FUS) is involved in mRNA transport and local translation, with roles in neuronal function and synaptic morphology.
  • FUS localizes to both the nucleus and synapses in neurons.
  • Many dendritic mRNAs form G quadruplex structures in their 3'-untranslated regions (3'-UTR).

Purpose of the Study:

  • To investigate the hypothesis that FUS recognizes G quadruplex structures in neuronal mRNA targets.
  • To explore the interaction between FUS and specific neuronal mRNAs, PSD-95 and Shank1.
  • To elucidate a novel mechanism for FUS-mediated neuronal mRNA targeting.

Main Methods:

  • Native gel electrophoresis to assess binding.
  • Steady-state fluorescence spectroscopy to quantify binding affinity.
  • Analysis of FUS C-terminal RGG box and full-length FUS interactions with RNA G quadruplexes.

Main Results:

  • Demonstrated specific nanomolar binding of FUS to RNA G quadruplex structures in the 3'-UTRs of PSD-95 and Shank1 mRNAs.
  • Confirmed binding by both the FUS RGG box and full-length FUS.
  • Identified a novel mechanism for FUS targeting of neuronal mRNAs.

Conclusions:

  • FUS specifically binds to G quadruplex structures in the 3'-UTRs of synaptic mRNAs like PSD-95 and Shank1.
  • This interaction suggests a new pathway for FUS in regulating neuronal mRNA.
  • The findings raise the possibility of FUS and FMRP cooperating in the translational control of these key synaptic genes.