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CircRNA_103762 promotes multidrug resistance in NSCLC by targeting DNA damage inducible transcript 3 (CHOP)

Guanhua Xiao1, Wenqi Huang1, Yongzhong Zhan1

  • 1Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Journal of Clinical Laboratory Analysis
|March 3, 2020
PubMed
Abstract

Insights

Circular RNA_103762 is elevated in non-small cell lung cancer (NSCLC) and promotes chemotherapy resistance by inhibiting CHOP expression. Downregulating circRNA_103762 may offer new therapeutic strategies for NSCLC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in various cancers, including non-small cell lung cancer (NSCLC).
  • The specific role of circRNA_103762 in NSCLC and its association with chemotherapy resistance warrants investigation.

Purpose of the Study:

  • To investigate the expression of circRNA_103762 in NSCLC.
  • To determine the correlation between circRNA_103762 and multidrug resistance (MDR) in NSCLC.
  • To elucidate the underlying mechanism involving CHOP.

Main Methods:

  • Quantitative real-time PCR (RT-PCR) to measure circRNA_103762 and CHOP expression.
  • Cell Counting Kit-8 (CCK8) assay to assess cell proliferation and drug sensitivity (IC50).
  • Migration and invasion assays to evaluate cellular motility.

Main Results:

  • CircRNA_103762 expression was significantly upregulated in NSCLC tissues and cells.
  • Knockdown of circRNA_103762 suppressed NSCLC cell proliferation, migration, and invasion.
  • Downregulation of circRNA_103762 increased CHOP expression and reduced multidrug resistance in NSCLC.

Conclusions:

  • CircRNA_103762 acts as an oncogene in NSCLC, promoting tumor progression.
  • CircRNA_103762 enhances MDR in NSCLC by suppressing CHOP expression.
  • Targeting circRNA_103762 could represent a novel therapeutic approach for overcoming chemotherapy resistance in NSCLC.
Keywords:
CHOPMDRNSCLCcircRNA_103762

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