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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

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Updated: Dec 27, 2025

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

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Different culture method changing CD105 expression in amniotic fluid MSCs without affecting differentiation ability

Ding Wang¹, Nengqing Liu¹, Yingjun Xie¹

¹Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Experimental Department of Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Journal of Cellular and Molecular Medicine

|March 3, 2020

Summary

This summary is machine-generated.

Mesenchymal stem cells (MSCs) from amniotic fluid show greater differentiation and immunosuppression than umbilical cord MSCs. Culture medium impacts proliferation and markers but not differentiation or immune effects.

Keywords:

CD105 amniotic fluid MSCs cell culture differentiation ability immune function

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Area of Science:

Stem cell biology
Immunology
Regenerative medicine

Background:

Mesenchymal stem cells (MSCs) are crucial regulators in physiological and pathological processes.
MSCs can be isolated from various tissues, including amniotic fluid and umbilical cord.
Understanding MSC properties is vital for therapeutic applications.

Purpose of the Study:

To compare the differentiation potential and immunosuppressive effects of amniotic fluid-derived MSCs (AF-MSCs) with umbilical cord-derived MSCs (UC-MSCs).
To evaluate the impact of different culture media on AF-MSC characteristics.
To assess the suitability of AF-MSCs as an alternative to UC-MSCs.

Main Methods:

Isolation and culture of MSCs from amniotic fluid and umbilical cord.
Comparative analysis of proliferation, morphology, cell surface markers, and mRNA expression.
Assessment of osteogenic and chondrogenic differentiation capabilities.
Evaluation of immunosuppressive effects on PHA-activated peripheral blood mononuclear cells (PBMCs).

Main Results:

AF-MSCs exhibited nearly all characteristics of UC-MSCs.
AF-MSCs demonstrated enhanced osteogenic and chondrogenic differentiation compared to UC-MSCs.
AF-MSCs showed a stronger immunosuppressive effect on PBMC proliferation.
Culture with commercial AFC medium improved CD105 expression, cell isolation, marker retention, and proliferation.

Conclusions:

AF-MSCs possess advantages over UC-MSCs in differentiation and immunosuppression.
Culture media influence MSC proliferation and surface marker expression but not differentiation or immune function.
AF-MSCs represent a promising alternative cell source for therapeutic applications.