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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

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The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
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IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

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When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
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IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

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A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
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Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.4K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

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Single beam low frequency 2D Raman spectroscopy.

Ilan Hurwitz, Dekel Raanan, Liqing Ren

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    |March 4, 2020
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel low-frequency two-dimensional Raman spectroscopy technique. It successfully reveals vibrational coupling dynamics in liquid tetrabromoethane at unprecedented low frequencies.

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    Area of Science:

    • Physical Chemistry
    • Spectroscopy
    • Materials Science

    Background:

    • Low frequency Raman spectroscopy probes collective molecular motions.
    • Accessing low frequencies (below 100 cm⁻¹) is difficult for multidimensional vibrational spectroscopy.
    • Understanding low-frequency dynamics is crucial for complex molecular structures.

    Purpose of the Study:

    • To develop a new method for measuring low-frequency two-dimensional (2D) Raman spectra.
    • To explore vibrational coupling dynamics in the low-frequency regime.
    • To provide a tool for deeper investigation of vibrational energy surfaces.

    Main Methods:

    • A novel 2D Raman spectroscopy scheme was developed.
    • The technique utilizes a spectrally shaped pump pulse and a transform-limited probe pulse.
    • Pulses are distinguished by their polarization states to enable measurement.

    Main Results:

    • Low-frequency 2D Raman spectra of liquid tetrabromoethane were successfully obtained.
    • Vibrational coupling dynamics were observed at frequencies as low as 115 cm⁻¹.
    • Experimental results were validated by numerical simulations.

    Conclusions:

    • The new method enables high-resolution measurements in the challenging low-frequency spectral region.
    • This technique provides new insights into the vibrational dynamics of molecular systems.
    • The method facilitates the exploration of vibrational energy landscapes in complex molecules.