Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pharmacodynamics: Overview and Principles01:21

Pharmacodynamics: Overview and Principles

2.7K
Pharmacodynamics is a scientific field that delves into drugs' intricate biochemical, cellular, and physiological effects on the human body. The study of pharmacodynamics helps us understand how drugs interact with the body and elicit various responses.
Most drugs' effects result from their interactions with drug receptors or targets within the body. These interactions trigger specific responses at the cellular or systemic level. Drug receptors can be found on the surfaces of cells or...
2.7K
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

9.9K
Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
9.9K
Principles of Drug Action01:24

Principles of Drug Action

7.8K
Drugs are chemical substances that modify biological responses by interacting with macromolecular targets such as receptors, ion channels, transporters, and enzymes. Pharmacodynamics describes the course of action of drugs leading to the physiological effect at a specific site in the body.
Drugs can be agonists or antagonists. Like the endogenous ligands, agonists always bind and activate the target to produce a cellular response. Agonist binding induces a conformational change which in turn...
7.8K
Drug Administration and Therapy Phases: Overview01:26

Drug Administration and Therapy Phases: Overview

1.1K
Drugs, the chemical agents used in diagnosing, treating, or preventing diseases, undergo a four-phase process of development: pharmaceutic, pharmacokinetics, pharmacodynamics, and therapeutic.
The pharmaceutical phase focuses on leveraging the physicochemical properties of the drug to design and manufacture an effective product. Variants include orally administered tablets or capsules, topical creams or ointments, and parenteral-delivery solutions or emulsions.
The pharmacokinetic phase...
1.1K
Biopharmaceutics and Pharmacokinetics: Overview01:28

Biopharmaceutics and Pharmacokinetics: Overview

3.1K
Understanding drugs, drug products, and their performance in pharmaceutical science is pivotal. Drugs, whether simple molecules or complex compounds, are designed to interact with the body's biological systems to diagnose, treat, or prevent diseases. Drug products include various delivery systems such as tablets, capsules, injections, and inhalers. The performance of these drug products is gauged by their ability to deliver the active ingredient to the desired site of action at the...
3.1K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pseudokinase-converting mutation in protein kinase C alpha drives chordoid glioma by pathway rewiring.

Proceedings of the National Academy of Sciences of the United States of AmericaĀ·2026
Same author

Protein kinase C theta: evolution, regulation, and function.

The Biochemical journalĀ·2026
Same author

Sex-specific disruptions in PKCĪ³ signaling in a mouse model of spinocerebellar ataxia type 14.

JCI insightĀ·2026
Same author

C-spine mutations of protein kinase C and Akt as a novel generalizable approach to create stable pseudokinases.

The Journal of biological chemistryĀ·2025
Same author

A PKCĪ· missense mutation enhances Golgi-localized signaling and is associated with recessively inherited familial Alzheimer's disease.

Science signalingĀ·2025
Same author

A Catalytically Inactive Protein Kinase C alpha Mutation Drives Chordoid Glioma by Pathway Rewiring.

bioRxiv : the preprint server for biologyĀ·2025

Related Experiment Video

Updated: Dec 27, 2025

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches
06:19

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches

Published on: June 16, 2023

3.7K

Pharmacology on Target.

Agnieszka T Kawashima1, Alexandra C Newton2

  • 1Department of Pharmacology, University of California at San Diego, San Diego, CA 92093, USA; Biomedical Sciences Graduate Program, University of California at San Diego, San Diego, CA 92093.

Trends in Pharmacological Sciences
|March 4, 2020
PubMed
Summary

Researchers developed a new tool to precisely target small-molecule inhibitors to specific locations within cells. This innovation allows for a more detailed study of localized kinase activity, overcoming limitations of global inhibitor distribution.

Keywords:
SNAP-tagkinase inhibitorsmitosisprotein kinases

More Related Videos

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction
05:28

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

17.4K
Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
13:18

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

1.6K

Related Experiment Videos

Last Updated: Dec 27, 2025

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches
06:19

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches

Published on: June 16, 2023

3.7K
A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction
05:28

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

17.4K
Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
13:18

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

1.6K

Area of Science:

  • Cell biology
  • Molecular pharmacology
  • Biochemistry

Background:

  • Small-molecule inhibitors are crucial for studying cell signaling pathways.
  • Current inhibitors distribute globally within cells, limiting the study of localized signaling events.
  • Understanding subcellular signaling is essential for dissecting complex biological processes.

Purpose of the Study:

  • To develop a novel tool for localizing small-molecule inhibitors to specific subcellular locations.
  • To enable the investigation of kinase activity at distinct protein scaffolds.
  • To overcome the limitations of global inhibitor distribution in cell signaling research.

Main Methods:

  • Design and implementation of a novel tool for targeted inhibitor localization.
  • Utilizing specific protein scaffolds for inhibitor anchoring.
  • Developing methods to assess localized kinase activity.

Main Results:

  • Successful localization of small-molecule inhibitors to designated protein scaffolds.
  • Demonstration of the tool's capability to study localized kinase activity.
  • Opening new avenues for dissecting subcellular signaling dynamics.

Conclusions:

  • The developed tool provides a refined approach to study localized cell signaling.
  • Targeted inhibitor localization enhances the understanding of kinase function in specific cellular compartments.
  • This strategy represents a significant advancement in molecular pharmacology and cell biology research.